The mouse embryonic yolk sac is an extraembryonic membrane that plays a role in hematopoietic circulation during the foetal stage. It is made up of a visceral yolk sac and a parietal yolk sac. Using a variety of molecular markers, the current study examined the normal development of VYS and PYS tissues in mice and developed a novel in vitro VYS cell culture system for evaluating VYS cell differentiation potentials. During development, gene expression in VYS and PYS tissues was analyzed using RT-PCR and immune histochemistry, and several useful markers for their identification were found: For VYS epithelial cells, HNF1, HNF4, Cdh1, Krt8, and Krt18, and for PYS cells, Stra6, Snail1, and vimentin. Gene expression and morphology in PYS cells were typical of mesenchymal cells. The number of HNF1-, HNF4-, E-cadherin-, and cytokeratin-positive VYS epithelial cells significantly decreased when VYS cells at 11.5 days of gestation were cultured in vitro for 7 days. Instead, the number of Stra6- and vimentin-positive PYS-like cells increased with culture. In addition, RT-PCR analyses revealed that, in the primary culture of VYS cells, gene expression of PYS markers increased while gene expression of VYS markers decreased. The fact that VYS epithelial cells rapidly transdifferentiate into PYS cells with mesenchymal characteristics in vitro is supported by these findings. As a result, a culture system that is suitable for investigating the molecular mechanisms underlying VYS Trans differentiation into PYS cells and the epithelial-mesenchymal transition may be developed. The YST was a solid mass in the pancreatic tail that measured 11 centimeters. The tumor had a variety of growth patterns, including microcystic reticular, endodermal sinus, and hepatoid, as well as medullary proliferation of tumor cells. The tumor cells expressed Sall4, glypican-3, and alpha-fetoprotein in an immunohistochemical test.

With Regards,
Sara Giselle
Associate Managing Editor
 Journal of Critical Care Obsestrics & Gynocology